FIG. 5.
TraMR1 interacts with TraDF. Coimmunoprecipitation of TraMR1 with TraDF-His (lane 1) and His-TraDF (lanes 3, 5, and 7) was performed as described in Materials and Methods. (A) Detection of TraMR1. Precipitation was strong with His-TraDF but weak with TraDF-His. Triton X-100 (lane 7) and EDTA (lane 5) had no influence on this interaction. Purified TraMR1 was used as a positive control (lane 9). (B) Detection of His-TraDF and TraDF-His. TraD could be detected in lanes 1, 3, 5, and 7, showing that TraD variants were present in approximately equal amounts in all of the experiments. Lack of a signal in lanes 2, 4, 6, and 8 shows that neither TraD nor TraM interacted with the beads used in the experiment. α-His AB, anti-His antibody.