TABLE 1.
Strains, plasmids, and phage used in this study
| Strains, plasmid, or phage | Description | Reference or source |
|---|---|---|
| Strains | ||
| J5 | pro met λ+ | IMBMa collection |
| MC1061 | araD139 Δ(ara leu)7697 ΔlacX74 galE15 galK16 hsdR2 (rK− mK+) rpsL (Strr) mcrA mcrB1 | IMBM collection; 5 |
| BL21(DE3) | E. coli B ompT dcm hsdS gal λ− (DE3) | Stratagene |
| SCS1 | endA1 hsdR17 (rK− mK+) supE44 thi-1 recA1 gyrA96 relA1 | Stratagene |
| UG10 | lon::Tn10 hsdR (rK− mK+) tre thi lac rpsL (Strr) | IMBM collection |
| XL1-Blue | endA1 hsdR17 (rK− mK+) supE44 thi-1 recA1 gyrA96 relA1 Δ(lac) [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene |
| Plasmids | ||
| R1-16 | Derepressed, deletion-containing variant of IncFII plasmid R1 Kmr | IMBM collection |
| pCALn | ColE1 ori PT7/TT7 Amprcbp tag | Stratagene |
| pMTD11 | pCALn Δ(BamHI-HindIII) Ω(traD11) Ampr | This study |
| pMTD21 | pCALn Δ(BamHI-HindIII) Ω(traD21) Ampr | This study |
| pBTD15 | pCALn Δ(BamHI-HindIII) Ω(traD15) Ampr | This study |
| pGZ119EH | ColD replicon; Ptaq/lacIq Cmr | 24 |
| pGTD15 | pGZ119EH Δ(XbaI-HindIII) Ω(cbp-traD15) Cmr | This study |
| pMALc | pBR322ori M13ori Ptaq/Trm/lacIq Amprmbp tag | New England Biolabs |
| pMALcTraMwt | pMALc Δ(XmnI-HindIII) Ω(traM amino acids 2-127) Ampr | This study |
| pSK410ch | pMS470Δ8 Δ(NdeI-HindIII) Ω(traD-His6) Ampr | 38 |
| pSK410nh | pMS470Δ8 Δ(NdeI-HindIII) (his6-traD) Ampr | 38 |
| pBR111 | Tcr pBR322 traM R1 | 20 |
| pGK111 | Ampr pUC119 traM R1 | 20 |
| pSU2007 | Kmr derivative of IncW conjugative plasmid R388; 2.5-kb EcoRI-SalI fragment exchanged for 1.5-kb EcoRI-SalI fragment carrying Kmr-encoding gene of Tn5 | 29 |
| Phage R17 | Male-specific RNA phage | IMBM collection |
a
IMBM, Institut für Molekular Biologie, Biochemie und Mikrobiologie.