FIG. 4.

Time courses of in situ deamination of ethanolamine by recombinant E. coli. Permeabilized cells of E. coli carrying pUCEA3 (6.6 × 10⁶ cells) (A) or pUCEA2 (1.1 × 10⁶ cells) (B) were incubated at 37°C for the indicated times in the following mixtures: 30 mM potassium phosphate buffer (pH 8.0), 50 mM KCl, 10 mM ethanolamine, 15 μM AdoCbl, in the presence (solid circles) or absence (open circles) of ATP plus MgCl₂ (3 mM each), in a total volume of 1.0 ml. The amount of acetaldehyde formed was determined as described in the text.