FIG. 1.

(A) Schematic illustration of the crl operon of M. pneumoniae. The four rectangular bars indicate the p65, hmw2, p41, and p24 genes in this operon. These genes are also designated MPN309, MPN310, MPN311, and MPN312 according to the serial numbering system of the M. pneumoniae genome project (8). The triangle and the square represent the p65 promoter (P_p65) and the terminator (t) of this operon, respectively. The figure is not drawn precisely to scale. (B) Structures of modified staphylococcal transposon Tn4001mod vectors (11, 22). Plasmid pISM2062.2 carries a Gm^r version of Tn4001mod. Plasmid pKV104 carries a Cm^r version of Tn4001mod. The IS256L, IS256R, and drug resistance genes (Gm^r and Cm^r) of Tn4001mod are illustrated. Cloning sites (Sm, SmaI; Ba, BamHI) in Tn4001mod are indicated. The eyfp fusion genes were inserted into the SmaI site of pISM2062.2. The ecfp fusion genes were inserted into the SmaI site of pKV104. The arrows indicate the directions in which the fusion genes were inserted.