FIG. 4.

Gliding motility of M. pneumoniae cells whose attachment organelles are fluorescently labeled with the EYFP-P65 fusion. Strain TK162 was observed by phase-contrast microscopy and fluorescence microscopy at 37°C. The phase-contrast image was recorded continuously with a video recorder. The microscope was shifted to the fluorescence setup for 2 s at 28-s intervals. The time intervals between images in this figure are 5 s. The positions of attachment organelles of four typical cells are indicated by colored arrowheads. The tracks of cell movement (positions of attachment organelles) are shown by colored lines in the bottom right panel (T). Bar, 5 μm.