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Mutant RsbRs (labeled at the tops of the gels) were mixed with RsbS and RsbT, and the mixture was applied to an analytical gel filtration column (Superose-12; Pharmacia). The first fractions (numbered 1 to 5), which contain the high-molecular-weight complex formed by RsbR and RsbS, were analyzed by SDS-PAGE to reveal whether RsbT was present. Table 1 shows the presence (+) or the absence (−) of RsbT in the different combinations of complexes of RsbS and RsbR or mutant RsbRs.
