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Effect of the phosphorylation of RsbR T205 or T171 on the phosphorylation rate of RsbS by RsbT. RsbR-RsbS complexes formed with wild-type RsbR (⧫) or wild-type RsbR-P (□) (A), RsbRT205A (•) or RsbRT205A-P (○) (B), and RsbRT171N (▴) or RsbRT171N-P (▵) (C) were mixed with RsbT and [γ-32P]ATP. Samples taken at the times indicated on the graphs were subjected to SDS-PAGE, and gels were exposed to a phosphor screen.
