FIG. 3.
The cqs locus in V. harveyi. (A) CAI-1 activity is shown for recombinant E. coli DH5α strains carrying either the cosmid pLAFR or pLAFR containing vibrio DNA. pJMH17F7 carries 25 kb of V. harveyi genomic DNA containing the gene responsible for CAI-1 activity. pJMH159, pJMH163, and pJMH164 contain Tn5lacZ insertions in pJMH17F7. pJMH273 carries only the cqsAVh gene, and pJMH282 carries only the cqsSVh gene. (B) Map of the cqs locus of V. harveyi and V. cholerae. Black triangles indicate the sites of Tn5lacZ insertions in pJMH163, pJMH164 and pJMH159. The cqsS and cqsA genes are shown in black and white, respectively. The downstream ORF in V. harveyi has amino acid homology to VCA0528 and is shown in gray. This gene was not fully sequenced. The downstream ORF in V. cholerae, denoted VCA0524, is shown in stripes.