FIG. 2.

The effect of a positive control substitution on the binding of MelR and RNA polymerase to the melAB promoter in vivo. Shown are the results of a ChIP experiment designed to monitor the effects of the MelR DK261 positive control substitution on the binding of MelR and RNA polymerase to the melAB promoter. The top panel is a gel on which PCR products, generated with melA primers and the anti-MelR, anti-RNA polymerase, or no-antibody control immunoprecipitate, were analyzed. The middle and bottom panels show the results of PCRs where the immunoprecipitates were reanalyzed by using primers to detect the glp or yabN genes.