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. 2004 Oct;186(20):6792–6799. doi: 10.1128/JB.186.20.6792-6799.2004

FIG. 1.

FIG. 1.

Restriction maps and Southern blot of GPL locus of Myco657 (Δrmt4) and Myco694 (Δrmt2). (A) Genetic arrangement of part of the GPL locus containing rmt2, rmt4, rmt3, and fmt. Positions of the BglII site are shown. The gene names used previously are shown in parentheses. The position where aph was inserted is designated kan. (B) Southern blot of BglII-digested genomic DNA extracted from M. smegmatis mc2155, Myco657 (Δrmt4), and Myco694 (Δrmt2). The blot was probed with digoxigenin-labeled DNA corresponding to rmt4. Gene replacement with aph introduced an additional BglII site, resulting in smaller hybridizing bands for the deletion mutants.