FIG. 3.
Monosaccharide analysis of mutant GPLs. Based-treated GPLs from wild-type and mutant cell lines were subjected to acid hydrolysis, and released monosaccharides were analyzed as their corresponding alditol acetate derivatives by GC-MS. (A) M. smegmatis mc2155, (B) Δrmt4, (C) Δrmt4 pHBJ415, (D) Δrmt2, and (E) Δrmt2 pHBJ409. dTal and methylated Rha alditol acetates were identified based on their retention time compared to authentic standards and their mass spectrum. Diagnostic ions for methylated Rha derivatives were as follows: 3-O-Me-Rha, m/z 101, 130, 143, 190, 203; 2,3-di-O-Me-Rha, m/z 102, 118, 143, 203; 3,4-di-O-Me-Rha, m/z 89, 131, 190; and 2,3,4-tri-O-Me-Rha, m/z 102, 118, 131, 162, 175. Asterisks indicate reagent peaks.