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. 2004 Oct;186(20):6792–6799. doi: 10.1128/JB.186.20.6792-6799.2004

FIG. 5.

FIG. 5.

HPTLC analysis of base-treated GPL extracts from M. smegmatis mutants transformed with M. avium methyltransferase genes. Lanes 1, host strain Myco29 (rmt3::Tn611); lanes 2, Myco493 fmt::str; lanes 3, Myco657 (Δrmt4); and lane 4, Myco694 (Δrmt2). Plasmids containing M. avium putative methytransferase genes (listed in parentheses after the plasmid) were introduced into each strain. (A) Strain transformed with pHBJ475 (mtfA); (B) strain transformed with pHBJ512 (mtfB); (C) strain transformed with pHBJ505 (mtfC); (D) strain transformed with pHBJ502 (mtfD). The “mutants” panel shows extracts from untransformed mutants and the parent strain (lane wt). Lanes showing a significant difference from the untransformed control are indicated with arrows.