TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant feature | Source |
|---|---|---|
| Strains | ||
| M. smegmatis | ||
| mc2155 | Parent strain used for mutagenesis | 14 |
| Myco29 | rmt3::Tn611, Kanr Strr | 9 |
| Myco493 | fmt::str | 7 |
| Myco657 | Δrmt4, Kanr | This study |
| Myco694 | Δrmt2, Kanr | This study |
| E. coli DH5α | Host for cloning | Stratagene |
| M. avium TMC 724 | Source of mtfA to -D | |
| Plasmids | ||
| pGEM-T Easy | Vector for efficient cloning of PCR products | Promega |
| pBluescript SK(+) | Cloning vector | Stratagene |
| pK18mobsacB | aph sacB | 13 |
| pK18mob | aph | 13 |
| pHBJ395 | pBluescript SK(+) containing aph | This study |
| pHBJ428 | pBluescript SK(+) containing str and sacB | This study |
| pHBJ430 | pHBJ428, Δrmt4 aph | This study |
| pHBJ488 | pHBJ428, Δrmt2 aph | This study |
| pVV16 | Mycobacterial-E. coli shuttle vector, hyg | T. Eckstein |
| pHBJ415 | pVV16, rmt4 | This study |
| pHBJ409 | pVV16, rmt2 | This study |
| pHBJ475 | pVV16, mtfA (M. avium) | This study |
| pHBJ512 | pVV16, mtfB (M. avium) | This study |
| pHBJ505 | pVV16, mtfC (M. avium) | This study |
| pHBJ502 | pVV16, mtfD (M. avium) | This study |