TABLE 2.

Oligonucleotide primers

Primer	Target or purpose	Sequence (5′-3′)
299	SOE to delete rmt4	CCCTCTAGACGTTTCACACTGACCGTAC
300	SOE to delete rmt4	CGTGCTTGGCACGGTAATGGCCATAACGTGCGCGTGCTCG
301	SOE to delete rmt4	ACGAGCACGCGCACGTTATGGCCATTACCGTGCCAAGCACG
302	SOE to delete rmT4	AGCTCTAGACAAACTGAGCTGATCATCG
310	sacB	GGGGTACCGCGTCGCTTGGTCGGTCA
382	sacB	TTGGTACCTTCTGAGCGGACTCT
383	rmt4	TTTGGATCCGACTGATCGCGATAGCCGA
384	rmt4	CCCGGATCCATCTCGGCGATCTTCACTC
388	rmt2	TTTGGATCCGGGGTGGGCTCGGTCGTC
389	rmt2	GCTGGATCCAATATATGAGCGGGCAGACC
428	PCR to delete rmt2	ATCTAGAGGGTTCCGGCCTCTCA
442	PCR to delete rmt2	TTTCTAGAGTGGTGGGTACAACGCAACTCCTC
443	PCR to delete rmt2	CTACATTCGTCGACTGGCCAAACCTCGGCTGTATCACCAAG
444	PCR to delete rmt2	ATACAGCCGAGGTTTGGCCAGTCGACGAATGTAGCGATGAA
458	mtfA	TGAATCGGTGGGCATTGTCG
459	mtfA	GTGCGGCACGGCGAGTTTG
496	mtfB	GGATCCGACTGATCGTGACATGCGATC
497	mtfB	AAGCTTGCGCCACAGCACGCCG
498	mtfC	GGATCCGACCGATCACGACACGCGG
499	mtfC	AAGCTTGGGCTTGCGCCACAGCACAC
500	mtfD	GGATCCGCACGCCGATGTTATGGTTG
501	mtfD	AAGCTTGCGGGCCGGCGTTGATGAG