TABLE 2.
Transcription from the promoter Pant in several strains
| Host straina | Genetic characteristics | Luciferase activity (RLU/ng)b
|
|
|---|---|---|---|
| Without anthranilate | With anthranilate | ||
| CA10 | ORF22+ ORF23+ | 4.21 ± 0.34 | 289.63 ± 25.08 |
| CA10dm1 | ORF22+ ORF23+ | 5.79 ± 0.94 | 279.45 ± 13.56 |
| CA10dm3 | ORF22− ORF23− | 2.94 ± 0.91 | 220.75 ± 13.06 |
| HS01 | ORF22+ ORF23+ | 0.33 ± 0.08 | 316.90 ± 30.94 |
| DS1 | ORF22− ORF23− | 0.48 ± 0.09 | 0.30 ± 0.03 |
| DS1(pMK2322) | ORF22+ ORF23+ | 2.78 ± 0.98 | 563.78 ± 7.97 |
| DS1(pMK23) | ORF22− ORF23+ | 2.70 ± 0.48 | 546.05 ± 61.98 |
| DS1(pMK22) | ORF22+ ORF23− | 0.79 ± 0.07 | 0.56 ± 0.06 |
| DS1(pMK) | ORF22− ORF23− | 1.03 ± 0.22 | 0.66 ± 0.15 |
a
P. resinovorans strains CA10, CA10dm1, and CA10dm3, and P. putida strains HS01 and DS1 were transformed by pBRCantA253. Strain DS1 was transformed by pBRCantA253 along with expression plasmids shown in parentheses. Luciferase activity was determined from the cells grown on SMM with or without anthranilate for 2 h. The values shown represent averages ± standard deviations of at least three independent experiments.
b
RLU, relative light units.