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cDNA synthesis in the presence of oligo(dT) primers or random hexamer primers with RNA isolated from PAO1. Lanes: 1, 100-bp DNA ladder; 2, cDNA mixture without RNA (negative control); 3, cDNA mixture without oligo(dT) primers (negative control); 4, cDNA preparation made by using oligo(dT) primers; 5, cDNA preparation made with random hexamer primers (positive control); 6, 100-bp DNA ladder. Preparations were run on a 2% agarose gel and stained with SYBR green I.
