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Primer extension inhibition (toeprint) assays using malE mRNA (0.2 pmol). High-salt-washed S. lividans 30S subunits (4 pmol) were used in all lanes; 4 pmol of each IF, when present, was preincubated with the 30S subunits (IF pre-3°) or were added to each reaction mixture after the ternary complex had been allowed to form (IF post-3°). The toeprint signal indicating a ternary complex with malE, 30S subunits, and tRNAfMet is marked with an arrowhead.
