FIG. 4.

Genes proximal to telomeres are downregulated in yaf9Δ and esa1-L254P strains. (A) Numbers of genes downregulated in yaf9Δ (red) and esa1-L254P (green) strains or upregulated in yaf9Δ (blue) and esa1-L254P (black) strains (compared to a WT strain) versus their distance from the telomere in 5-kb intervals up to 40 kb. Strains used were yaf9Δ YBC1582, esa1-L254P YBC1646, WT YBC1665 (for YBC1582), and WT YBC605 (for YBC1646). (B) As in panel A, but with the ordinate showing the fractions of genes downregulated in the interval. Ratios (yaf9Δ/WT transcript levels) for each gene from three independent transcription profiling experiments were averaged (see Materials and Methods). Genes that were downregulated >2-fold and located in the interval are represented.