FIG. 10.

Overexpression of STC2 protects cells from thapsigargin-induced cell death. (A) HeLa cells were transfected with empty vector or STC2 expression plasmid. Expression of STC2 was analyzed by Western blotting. (B and C) One day after transfection, cells were treated or not treated with thapsigargin (Tg) or tunicamycin (Tm) and then processed as described in Fig. 9E and F to quantify cell death. The graph shows the percentage of cell death expressed as means ± SE for three independent experiments. ✽, P < 0.05; ✽✽, P < 0.01.