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. 2004 Nov;24(21):9456–9469. doi: 10.1128/MCB.24.21.9456-9469.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 3. — UPR-mediated expression and secretion of STC2. (A) Specificity of STC2 antiserum. N2a cells were transfected with empty vector or plasmids encoding C-terminally tagged STC1 or untagged STC2. Aliquots of detergent lysates and conditioned media were analyzed by immunoblotting with STC2 antiserum or CT11 (tag) antibody. Note that STC2 antibody does not cross-react with STC1. (B) N2a cells were treated with tunicamycin or thapsigargin for the indicated periods, and detergent lysates were analyzed by blotting with STC2, BiP, CHOP, and β-actin antibodies. Secreted STC2 were immunoprecipitated with STC2 antiserum and then analyzed by immunoblotting with the same antibody. Note the UPR-mediated induction and secretion of STC2.