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. 2004 Nov;24(21):9682–9694. doi: 10.1128/MCB.24.21.9682-9694.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 4. — Visualization of GCRs by PFGE in a strain containing 10 marked Ty1s (LSY1259) after HO induction. The cells underwent a 60-min HO induction and were then plated on the noninducing medium. Three resulting individual colonies were used to prepare the DNA plugs (lanes 2 to 4). Lane 1 corresponds to the starting strain prior to HO induction. (Left) Pulsed-field gel stained with SYBR Gold. Arrows, chromosomes with an abnormal mobility; numbers on the left, chromosome numbers. (Right) Pulsed-field gel blotted and probed with HIS3. Numbers on the left, numbers of HIS3-marked Ty1s in the corresponding band in LSY1259; *, signal from the endogenous his3 gene.