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. 2004 Nov;24(21):9682–9694. doi: 10.1128/MCB.24.21.9682-9694.2004

FIG. 7.

FIG. 7.

Accumulation of ssDNA intermediates at MAT after phleomycin treatment. (Top) A 60-min HO induction was performed in two equivalent cultures of MRE11 (LSY678; left two panels) and mre11-D56N (LSY1032; right two panels) strains. Phleomycin was added to one culture of each strain during the entire period of HO induction, at a final concentration of 250 μg/ml (163 μM). ssDNA formation at MAT was analyzed by alkaline gel electrophoresis (see Materials and Methods). t, time in minutes between the beginning of HO induction and the harvest of the corresponding cells. Values for time zero for LSY678 with no phleomycin are not plotted because of partial DNA degradation. (Bottom) Quantitation of the ssDNA intensities, normalized with the LEU2 signal and expressed as the fraction of the maximum intensity of ssDNA1 for each strain. Error bars correspond to the range of the data from two independent experiments for mre11-D56N and the standard deviations of three independent experiments for MRE11.