TABLE 1.
Yeast strains
| Strain | Genotypea | Origin or reference |
|---|---|---|
| W1588-4C (LSY678) | MATa | R. Rothstein |
| W1588-4A (LSY679) | MATα | R. Rothstein |
| LSY723 | MATarad52::LEU2 met17::ADE2 | 5 |
| LSY779 | MATamre11::LEU2 | 45 |
| LSY894-2B | MATα met17-s ADE2 exo1::HIS3 | 60 |
| LSY894-4C | MATamet17-s ADE2 exo1::HIS3 | 60 |
| LSY1032 | MATamre11-D56N | Transformation of W1588-4C with pSM444 |
| LSY1159 | MATa 2 Ty1-HOcs-HIS3 | pBL001 retrotransposition in W1588-4C |
| LSY1164 | MATa 3 Ty1-HOcs-HIS3 | pBL001 retrotransposition in LSY1159 |
| LSY1168 | MATa 4 Ty1-HOcs-HIS3 | pBL001 retrotransposition in W1588-4Cb |
| LSY1170 | MATa 1 Ty1-HOcs-HIS3 | pBL001 retrotransposition in W1588-4C |
| LSY1173 | MATa 3 Ty1-HOcs-HIS3 | pBL001 retrotransposition in LSY1170 |
| LSY1216 | MATa 4 Ty1-HOcs-HIS3 | pBL001 retrotransposition in LSY1164 |
| LSY1220 | MATα 4 Ty1-HOcs-HIS3 | LSY1216 × W1588-4A spore |
| LSY1223 | MATa 6 Ty1-HOcs-HIS3 | pBL001 retrotransposition in LSY1173 |
| LSY1228 | MATa 6 Ty1-HOcs-HIS3 | pBL001 retrotransposition in LSY1168 |
| LSY1240 | MATα 8 Ty1-HOcs-HIS3 | LSY1228 × LSY1220 spore |
| LSY1248 | MATa 10 Ty1-HOcs-HIS3 | pBL001 retrotransposition into LSY1223 |
| LSY1259 | MATα 10 Ty1-HOcs-HIS3 | pBL001 retrotransposition in LSY1240 |
| LSY1264 | MATα 9 Ty1-HOcs-HIS3 | LSY1248 × LSY1240 spore |
| LSY1285 | MATα 10 Ty1-HOcs-HIS3 mre11-D56N | Derived from LSY1259 by transformation with pSM444 |
| LSY1299 | MATα 9 Ty1-HOcs-HIS3 mre11-D56N | Derived from LSY1264 by transformation with pSM444 |
| LSY1477 | MATα 10 Ty1-HOcs-HIS3 rad52::LEU2 | Derived from LSY1259 by transformation with pSM20 |
| LSY1481 | MATα 9 Ty1-HOcs-HIS3 rad52::LEU2 | Derived from LSY1264 by transformation with pSM20 |
| LSY1483 | MATα 10 Ty1-HOcs-HIS3 mre11-D56N rad52::LEU2 | Derived from LSY1285 by transformation with pSM20 |
a
All the strains are of the W303 genotype (leu2-3,112 trp1-1 ura3-1 can1-100 ade2-1 his3-11,15 RAD5); only the mating type and differences from this genotype are shown.
b
Three rounds of retrotransposition were performed before analysis.