FIG. 10.
Disturbance of the actin cytoskeleton induces c-Jun. (A) Effect of increased cofilin on c-Jun. Left panel, HeLa cells were transfected with pRK5 cofilin (+) or vector control (−), and c-Jun levels were detected by Western blotting of total cell extracts. Right panel, HeLa and c1 cells were compared. Representative blots are shown. (B) Effect of increased actin or the G-actin mutant G13R on c-Jun. Lysates from the experiment described for Fig. 9B were analyzed for c-Jun protein levels by Western blotting. Representative blots are shown. wt, wild type.