FIG. 6.
The GR inhibitory effect of cofilin does not require physical interaction. (A) Coimmunoprecipitation of GR and cofilin. HEK cells were transfected with 10 μg of cofilin-flag or flag-GR. After precipitation, blots were probed with antibodies directed against cofilin, GR, actin, and hsp90. Representative blots are shown. (B) GST pull-down assay with GR and cofilin. GST-GR was expressed in bacteria and immobilized to glutathione-Sepharose beads. In vitro-translated 35S-cofilin was added to the beads, and eluates were tested for the presence of cofilin and hsp90 as a positive control. +, present; −, absent.