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. 2004 Nov;24(21):9371–9382. doi: 10.1128/MCB.24.21.9371-9382.2004

FIG. 9.

FIG. 9.

Disruption of the actin cytoskeleton or increase in G-actin reduces GR function. (A) Effect of cytochalasin B on intracellular distribution of GR (left panel) and transcriptional activity (right panel). For translocation assays, cells were transfected and evaluated as described for Fig. 8B, except that they were treated with cytochalasin B for 1 h before fixing. For luciferase assays, HEK cells were transfected with MTV-Luc, pCMVβ-Gal, pRK7GR, and pRK5cof, treated with cytochalasin B for 1 h, and stimulated with 1 μM dex for 24 h in the continued presence of cytochalasin B. Inducibility with empty vector was set to 100%. Data represent the means + standard errors of the means of the results for six independent experiments. (B) Effect of transient wild-type and mutant actin expression on intracellular distribution of GR (left panel) and transcriptional activity (right panel). For translocation assays, cells were transfected with pRK5actin, pRK5G13R, or empty vector and then treated and evaluated as described for Fig. 8B. For luciferase assays, HEK cells were transfected with MTV-Luc, pCMVβ-Gal, pRK7GR, and 5 μg of pRK5actin or pRK5G13R and stimulated with 1 μM dex for 48 h. Inducibility with empty vector was set to 100%. Data represent the means + standard errors of the means of the results for eight independent experiments.