TABLE 1.
List of clones isolated after mutagenesis and selection
| Clone | Mutagenic agent | Characteristic | Differentially expressed proteins |
|---|---|---|---|
| 1.3/1 | ICR191 | Mutation in TK gene or promoter | |
| 1.3/2 (mut1) | ICR191 | Mutation in trans | Thioredoxin (1), cofilin (2), hsp27 (3), reticulocalbin (4)a |
| 1.3/3 | ICR191 | Mutation in TK gene or promoter | |
| 1.3/4 | ICR191 | False positiveb | |
| 1.3/5 | ICR191 | False positive | |
| 1.3/6 | ICR191 | False positive | |
| 1.3/7 | ICR191 | False positive | |
| 1.4/1 | ICR191 | Mutation in TK gene or promoter | |
| 2.3 | ICR191 | False positive | |
| 3.3 | ICR191 | False positive | |
| 5.3/2 (mut2) | ICR191 | Mutation in trans or GR gene | Under investigation |
| ENU5 (mut3) | ENU | Mutation in trans or GR gene | Under investigation |
a
Numbers in parentheses refer to spots on the 2-D gels in Fig. 4.
b
False positive refers to clones that survived selection but did not show GC resistance, i.e., hormone was still able to induce the reporters.