FIG. 2.

Mutations of Gly⁴⁹³ in the conserved GPCWL motif in POL1 induce thermosensitivity in growth and cell cycle delay. (Left panel) Strain DFB (MATa pol1Δ ura3-52 leu2) carrying plasmid pRS315-POL1 or its mutant derivatives were grown on synthetic medium lacking leucine in 5-fold serial dilutions and incubated at 25, 30, and 34°C. (Right panel) Flow cytometry analyses of pol1 mutants containing mutations in the GPCWL motif. Each asynchronous strain was cultured exponentially at 25°C and then shifted to 30°C for 4 h.