FIG. 4.

Interaction of the N-terminal region of Pol1p with Spt16p is compromised in the pol1-1 mutant. (A) A schematic diagram of the N-terminal fragments of the wild type and Pol1p in the pol1-1 mutant used as bait. (B) Strain SKY48 was transformed in pairwise combinations of bait and prey vectors as described in Materials and Methods, and the transcriptional activation of LEU2 was scored by determining leucine prototrophy. (C) Transcriptional activation of lacZ was examined with an X-Gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside) overlay assay.