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. 2004 Nov;24(21):9401–9413. doi: 10.1128/MCB.24.21.9401-9413.2004

FIG. 3.

FIG. 3.

The rad62-1 mutant is defective in DNA repair. (A and B) Sensitivities of rad62-1 mutant to UV and gamma rays. The cells at mid-logarithmic phase were irradiated with UV or gamma rays at the indicated doses, and the relative plating efficiencies were determined. Wild-type strain, MMP1 (♦); rad62-1 strain, MMP2 (▪); rhp51Δ strain, MMPX3 (▴); rad62-1 rhp51Δ strain, MMPX4 (•). The data represent the averages of the results from three experiments. (C) The rad62-1 mutant is defective in repairing DNA DSBs. Genomic DNA of wild-type and rad62-1 strains was analyzed by PFGE. Samples were taken at the indicated times (in hours) after irradiation by gamma rays at 500 Gy (+) or before irradiation (−).