TABLE 2.
Plasmids used in this study
| Plasmid | Insertiona | Plasmid used for derivation | Source or reference |
|---|---|---|---|
| pSLR4 | rad62 genomic | pUC118 | This study |
| pSLR4D | rad62::ura4 | pSLR4 | This study |
| pREP41 | ars1 LEU2 nmt1* | 31 | |
| pREP41X | ars1 LEU2 nmt1* | 12 | |
| pBRC1 | ars1 LEU2 nmt1* brc1 cDNA | pREP41X | M. O'Connell |
| pRAD62 | ars1 LEU2 nmt1* rad62 cDNA | pREP41 | This study |
| pRAD60 | ars1 LEU2 nmt1* rad60 cDNA | pREP41 | This study |
| pREP41EGFP-N | ars1 LEU2 nmt1* EGFP | pREP41X | 9 |
| pGFP62 | ars1 LEU2 nmt1* EGFP-rad62 cDNA | pREP41EGFP-N | This study |
a
*, nmt medium-strength promoter.