FIG. 2.
Dna2 is required for the telomere length regulation. (A) The telomere length of the dna2-C2 mutant was analyzed by Southern hybridization analysis at the permissive (25°C) and semipermissive (30°C) temperatures. Lanes 1, 10, and 11, wild-type cells at 30°C (JY741); lane 2, dna2-C2 mutant at 25°C (HK10); lanes 3 to 7, dna2-C2 mutant incubated at 30°C (HK10) for the indicated (at the top) numbers of days; lane 8, dna2-C2 mutant incubated at 30°C for 25 days in an independent experiment (HK10); lanes 9 and 13, rad50-d cells at 30°C (KT120); lane 12, wild-type cells at 25°C (JY741); lane 14, rad50-d cells at 25°C (KT120). (B) Time course of the change of telomere length in dna2-C2 mutant after a temperature shift to the semipermissive temperature (30°C). The data shown in panel A were plotted.