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RNAi of the T. brucei TRF4. (A) Growth curve of the TRF4 RNAi cell line in the absence (−) or presence (+) of tetracycline (tet). (B) The TRF4 RNAi cell line was induced with tetracycline for the indicated number of hours, and poly (A+) RNA was probed for TRF4 mRNA. Ethidium bromide staining of rRNA served as a loading control.
