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. 2017 Jan 9;12(1):e0169385. doi: 10.1371/journal.pone.0169385

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© 2017 Mo et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A) 10-frame averaged ROI. (B) ROI with skeletonization superimposed in red. (C) Skeleton only. (D) Skeleton dilated to 15 μm in diameter. (E) Signal mask from dilated skeleton superimposed with the ROI. (F) Background mask from inverse of dilated skeleton superimposed with the ROI. Images were contrast-stretched for display purposes only.