. Author manuscript; available in PMC: 2017 Dec 1.

Published in final edited form as: Phytochem Rev. 2016 May 10;15(6):1035–1056. doi: 10.1007/s11101-016-9469-x

Table 2.

HPLC and HPTLC methods for analysis of nutmeg constituents in crude extracts and herbal preparations

Application	Technique/Method	Target Constituents	Ref.
Quality control of kernel and mace oil obtained by supercritical fluid extraction	RP HPLC-UV: Lichrosphere 100, C18, 125 × 4 mm, 5 ; gradient MeOH/ACN/0.01% aq. PA; 1 mL/min; 23 min; 265 nm	9 Phenylpropanoids: ele, eug, ieug, ielem, mxeug, myeug, myr, saf	(Ehlers et al. 1998)
Determination of bioactive constituents in crude extracts and market products of fruit	RP UPLC-UV: Acquity BEH, C18, 1.7, 50 × 2.1 mm; gradient MeOH/0.1% aq. FA (10–100%); 0.4 mL/min; 9.4 min; MRM MS pos-neg mode switching	16 compounds: apg, cafa, cat, fera, eug, ieug, irhm, kmf, myeug, ma, myr, ola, ursa, pca, qrc, trm	(Pandey et al. 2015)
Fingerprinting and guided isolation of kernel constituents	RP HPLC-UV: HyPurity, C18, 3, 150 × 4.6 mm; gradient ACN/0.1% FA; 1 mL/min; 30 min; 270 nm	13 compounds: acneol, dhb, elem, ielem, licA, licB, licC, malB, malC, mlicA, mlicB, myr, sur	(Chiu et al. 2016)
Pharmacognostical standardization of kernel extract and quality control of Indian products	NP HPTLC-UV: silica gel 60 F₂₅₄ coated plates; toluene:ethyl acetate (9:1); 254 nm; 366 nm; p-anisaldehyde/H₂SO₄	17 spots: Only myr identified	(Tripathi and Dwivedi 2015)

UHPLC: Ultra-performance liquid chromatography; HPTLC: high-performance thin-layer chromatography; NP: normal phase; RP: reversed phase; UV: ultraviolet detector; MS: mass spectrometry detector; acetoxyneolignan (51): acneol; apigenin: apg; caffeic acid: cafa; catechin: cat; dihydrobenzofuran (41): dhb; elemicin (10): elem; eugenol (13): eug; ferulic acid: fera; isoeugenol: ieug; isoelemicin: ielem; isorhamnetin: irhm; kaempferol: kmf; licarin A (35): licA; licarin B (36): lic B; licarin C (37): licC; malabaricone B (59): malB; malabaricone C (60): malC; 5-methoxylicarin A: mlicA; 5-methoxylicarin B (38): mlicB; methoxyeugenol: mxeug; methyleugenol (11): myeug; methylisoeugenol (14): myieug; myristic acid: ma; myristicin (9): myr; oleanolic acid: ola; ursolic acid: ursa; protocatechuic acid: pca; quercetin: qrc; safrole (12): saf; surinamensin (50): sur; trimyristin: trm; ACN: acetonitrile; MeOH: methanol; FA: formic acid; PA: phosphoric acid