Figure 2.

Topography of mitochondrial survivin. (A) Sensitivity to proteinase K. Mitochondrial or cytosolic fractions were treated with the indicated concentrations of proteinase K and analyzed by immunoblotting. Lower panel: Quantification of Bcl-2 or survivin proteolysis by proteinase K treatment. (B) Permeabilization of mitochondrial membrane. Mitochondrial fractions were treated with the indicated increasing concentrations of digitonin, and pellets (P) or supernatants (S) were analyzed by immunoblotting. mt-Hsp70, mitochondrial Hsp70.