. Author manuscript; available in PMC: 2017 May 1.

Published in final edited form as: J Immunol. 2016 Apr 1;196(9):3896–3909. doi: 10.4049/jimmunol.1502122

Table II.

GB1-mCRT(P), GB1-mCRT(C) and mCRT(1-351 ΔP)-mediated inhibition of mCRT(WT) interactions with live and apoptotic MEFs and murine peritoneal macrophages. With live MEFs, the data represent the mean ± SEM of 7 (for GB1-mCRT(P) and GB1-mCRT(C)) or 4 (for GB1-mCRT(1-351 ΔP)) independent measurements. With apoptotic MEFs, the data represent the mean ± SEM of 7 (for GB1-mCRT(C), 6 (GB1-mCRT(P)) or 3 (for GB1-mCRT(1-351 ΔP)) independent measurements. With macrophages, the data represent mean ± SEM of 5 (for GB1-mCRT(P)), 3 (for GB1-mCRT(C)) or 2 (mCRT(1-351 ΔP)) independent measurements, and inhibition by GB1-mCRT(C) in 2 of 5 measurements was insufficient for accurate IC₅₀ estimates. All inhibition studies were undertaken in the presence of 5 mM CaCl₂.

Inhibitor	IC₅₀ (μM) ^a
Inhibitor	Live MEFs	Apoptotic MEFs	Macrophages
GB1-mCRT(P)	18.09 ± 2.29	118.0 ± 33.9	15.76 ± 3.97
GB1-mCRT(C)	268.3 ± 109.4	23.49 ± 4.82	112.8 ± 14.13
mCRT(1-351 ΔP)	31.50 ± 11.62	110.0 ± 65.07	7.64 ± 0.38

Data represent Mean ± SEM