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The N-terminus targets HsPDF to the mitochondria. HeLa cells were transfected with full-length or N-terminally truncated HsPDF (AAs 64–244) tagged at the C-terminus with YFP and imaged alive by laser scanning confocal microscopy 24 hours after transfection and 30 minutes after addition of MitoTracker Red CMXRos to the media. Scale bar: 10 μm.
