Figure 3. Different resolvase-dependence of crossover formation at the two insert loci.

(A) Crossover frequencies (average of CO1 and CO2) measured as in Figure 2C from HIS4 insert-containing mutants lacking MutLγ (mlh3), structure-selective nucleases (mms4-md yen1 slx1) or both resolvase activities (mlh3 mms4-md yen1 slx1). (B) Crossover frequencies in URA3 insert-containing strains, measured as in panel A. Values are the average of two independent experiments; error bars represent range. (C) Final crossover levels (average of 8 and 9 hr values for two independent experiments), expressed as percent of wild type. Note that, in mlh3 mutants, crossovers in HIS4 inserts are reduced by nearly 60%, while crossovers in URA3 inserts are reduced by less than 10%. (D) Final noncrossover levels, calculated as in C, expressed as percent of wild type. Representative Southern blots are in Figure 3—figure supplement 2.

DOI: http://dx.doi.org/10.7554/eLife.19669.007

Figure 3—figure supplement 1. VDE-DSB and NCO frequencies in resolvase mutants.

(A) VDE-DSB frequencies (top), measured as in Figure 2B, and NCO frequencies (bottom), measured as in Figure 2C, from HIS4 insert-containing strains. (B) As panel A, with strains containing inserts at URA3. Data are the average of two independent experiments; error bars represent range. Representative Southern blots are in Figure 3—figure supplement 2.

DOI: http://dx.doi.org/10.7554/eLife.19669.008

Figure 3—figure supplement 2. Southern blots of HindIII and HindIII-VDE digests of DNA from HIS4 insert-containing strains (top) and from URA3 insert-contaning strains (bottom).

Probes and gel labels are as in Figure 1; JM—joint molecule recombination intermediates.

DOI: http://dx.doi.org/10.7554/eLife.19669.009