(
A) Cumulative DSB levels, expressed as loss of the VRS-containing insert, calculated as in
Figure 2A. (
B) Relative recovery of recombination products, calculated as in
Figure 2—figure supplement 1B. (
C) VDE-DSB frequencies, as in
Figure 2B. (
D) NCO frequencies, as in
Figure 2C. In all four panels, solid lines denote values from
spo11 strains; values from wild type (dotted lines, from
Figure 2 and
Figure 2—figure supplement 1) are presented for comparison. (
E) DSB (top) and NCO (bottom) frequencies in
spo11-Y135F strains with inserts at
HIS4. (
F) DSB (top) and NCO (bottom) levels in
spo11-Y135F strains with inserts at
URA3. For all panels, values are from two independent experiments; error bars represent range. Representative Southern blots are in
Figure 5—figure supplement 2.