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. 2017 Jan 3;25(1):107–120. doi: 10.1016/j.str.2016.11.015

Figure 2.

Figure 2

The (OL3:My4L)2 Complex Dimer

(A) In the crystal, two OL3:My4L heterodimers are arranged around a non-crystallographic two-fold axis (vertical in this view) forming a W-shaped (OL3:My4L)2 dimeric assembly. My4L and OL3 are in slate blue and green, respectively, with one heterodimer shown as a solid surface and the other in cartoon mode with a transparent surface. The dotted line at the C-terminus of L indicates the flexible region leading to My5 not visible in the structure.

(B) Superdex S75 10/300 SEC traces for the OL3:My4LMy5 complex injected in the column at 3 mg/mL (thin continuous line) and 8.2 mg/mL (thick continuous line). The OL3:My4LMy5 complex forms a mixture of monomers and dimers in a concentration-dependent manner. The elution profile of calibration markers is shown with a gray broken line. Molecular weights are indicated.