FIG. 1.

Colocalization of kinase-active but not wild-type c-Fes with MTs. Wild-type GFP-Fes (WT) or a kinase-active GFP-Fes mutant (L145P) was transiently expressed in COS-7 cells. Forty-eight hours after transfection, the cells were fixed, permeabilized, and immunostained with both α-tubulin and Fes-tyrosine phosphospecific antibodies. Secondary antibodies conjugated with Cy3 or Cy5 were used to differentiate tubulin from active Fes (pFes) staining by confocal microscopy. Overall Fes protein distribution was visualized by GFP fluorescence. A merged image is shown on the far right. GFP, Cy5, and Cy3 fluorescences are represented as green, blue, and red, respectively, while merged colors appear as white.