TABLE 3.
Primers used for site-directed mutagenesis of NIP1
| Primer | Sequence (5′ to 3′) |
|---|---|
| LVNIP1-AVAI | GCC TGC CAA GCC CGA GAT CTA |
| LVNIP1MUT | GCC AAG CCC GAG ATC TAC GCA TAT G |
| LV22 | CAT TTT CTC TAG AAA CTT |
| LVBOX1 | CCA AGC CCG AGA TCT ACG CAT ATG GCA GCT GCT GCA GCA GCA GCA GCA GCA TAC GAT GTA GCC AGT TCT TCA TCC GAA GAA |
| LVBOX2 | CCA AGC CCG AGA TCT ACG CAT ATG TCC CGT TTC TTT TCG TCT AAT TAC GAA GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA |
| LVBOX2C | GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA GAT CTT TTA TCT TCG TCT GAA |
| LVRBOX3B | TTC TTC GGA TGA AGA ACT GGC |
| LVBOX3C | GCC AGT TCT TCA TCC GAA GAA GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA TTA AGC TCT TCC TCC TCT GAG |
| LVRBOX4B | CAA ATC TTC TTC AGA CGA AGA |
| LVBOX4C | TCT TCG TCT GAA GAA GAT TTG GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA GAC CAA GAA TCT GAC GAC TCC |
| LVRBOX5B | CAA TTC AGA CTC AGA GGA GGA |
| LVBOX5C | TCC TCC TCT GAG TCT GAA TTG GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA GAA AGT GAA AGT GAA AGT GAA |
| LVRBOX6B | ATT GAA AAA GGA GTC GTC AGA |
| LVBOX6C | TCT GAC GAC TCC TTT TTC AAT GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA GAC TCT GAT GAT TCT GAT GCA |
| LVNBOX61-R | TCT GAC GAC TCC TTT TTC AAT CGT CGA CGA CGA CGA CGA CGA CGA CGA GTA GAC TCT GAT GAT TCT GAT |
| LVNBOXA2 | ATT GAA AAA GGA GTC GTC AGA |
| LVRBOX7B | TAC ATC AGC TTC ACT TTC ACT |
| LVBOX7C | AGT GAA AGT GAA GCT GAT GTA GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA GGT CCT GAC TGG TTC AAG AAA |
| LVRBOX8B | ATA AGG CTT TGC ATC AGA ATC |
| LVBOX8C | GAT TCT GAT GCA AAG CCT TAT GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA AGA AAA CAA GGT GGA GGT TCA |
| LVRBOX9B | GAA CTC AGA TTT CTT GAA CCA |
| LVBOX9C | TGG TTC AAG AAA TCT GAG TTC GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA TTG AAA AGC TCT AAC TAT GAT |
| LVRBOX10B | AAA TTT ATT TGA ACC TCC ACC |
| LVBOX10C | GGT GGA GGT TCA AAT AAA TTT GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA GAA GAA TCC GAT GAA GAA GAT |
| LVRBOX11B | ATC ACT GGA ATC ATA GTT AGA |
| LVBOX11C | TCT AAC TAT GAT TCC AGT GAT GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA GTA GTC AAG TCT GCC AAA GAA |
| LVRBOX12B | CTT CTT GCC ATC TTC TTC ATC |
| LVBOX12C | GAT GAA GAA GAT GGC AAG AAG GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA GAT GAA ATG CAA GAC GTT TAT |
| LVRBOX13B | CAA TAG TTT TTC TTT GGC AGA |
| LVBOX13C | TCT GCC AAA GAA AAA CTA TTG GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA TCT CAA GCT GAG AAC TCT GAT |
| LVRBOX14B | GAT CTT ATT ATA AAC GTC TTG |
| LVBOX14C | CAA GAC GTT TAT AAT AAG ATC GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA ACT ATT TCT AAT GAG TTT GAT |
| LVRBOX15B | CAA CCA GTC ATC AGA GTT CTC |
| LVBOX15C | GAG AAC TCT GAT GAC TGG TTG GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA CGT CTC TTA GTT AGG GCT CAA |
| LVRBOX16B | CGA GAT CAA ATC AAA CTC ATT |
| LVBOX16C | AAT GAG TTT GAT TTG ATC TCG GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA TGG GGG ACT CCA AAT ATT TTC |
| LV22-HIII | AAT ACA AGC TTA ACT TTC TTG ACT CTT TGC TT |
| LV91 | ATA TAG AGC TCT GAA AGG AAT GAA AAA TTA |
| LV92 | ATA TAG GTA CCT TTC GTA GAT CTC GGG CTT |
| LV101 | ATA TAG TCG ACC TAA GCA GGA GAG TAT AA |
| LV102 | ATA TAG CAT GCT GCA ACT GTT GTA TCT TG |