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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Dec 7;1863(2):569–584. doi: 10.1016/j.bbadis.2016.12.005

Fig. 9.

Fig. 9

Immunohistochemical analysis of E-selectin and VCAM-1 protein expression in mouse colon tissues. As whole tissues are not ideal for analyzing endothelial cell specific gene expression, we performed IHC to analyze adhesion molecules E-selectin and VCAM-1 protein expression in ECs within the tissue. GPR4 KO-DSS mice have reduced E-selectin and VCAM-1 protein expression in colonic mucosal vasculature when compared to WT-DSS mice. E-selectin expression could be visualized as brown signals in (A) WT-control, (B) WT-DSS, (C) GPR4 KO-control, and (D) GPR4 KO-DSS colon tissues. VCAM-1 expression could be visualized in (E) WT-control, (F) WT-DSS, (G) GPR4 KO-control, and (H) GPR4 KO-DSS colon tissues. 40× microscope objective. Red Arrows indicate blood vessels.