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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Mol Cancer Ther. 2016 Oct 28;16(1):35–44. doi: 10.1158/1535-7163.MCT-16-0186

Figure 3. Exogenous AKR1C3 promotes testosterone production and regulates AR transcriptional activity in prostate cancer.

Figure 3

A. LNCaP-neo and LNCaP-AKR1C3 cells were cultured in serum free, phenol red free RPMI1640 medium for 3 days. One hundred million cells were collected per group and testosterone level was examined by LC-MS. B. LNCaP-neo and LNCaP-AKR1C3 cells were cultured in CS-FBS conditions for 3 days, total RNA was extracted and PSA mRNA levels were examined by qRT-PCR, C. The supernatants were collected and PSA level was examined by PSA ELISA. D. LNCaP-neo and LNCaP-AKR1C3 cells were cultured in CS-FBS conditions for 3 days, whole cell lysates were subjected to ChIP assay. *p<0.05.