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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Cancer Discov. 2016 Sep 23;7(1):86–101. doi: 10.1158/2159-8290.CD-16-0127

Figure 1. Loss of Keap1 or Trp53 promotes airway basal stem cell self-renewal in vitro.

Figure 1

(A) Relative number of primary tracheospheres formed by wild type (WT) or Keap1-deleted mouse tracheal epithelial cells (N=4).

(B) Relative number of secondary tracheospheres formed by WT or Keap1-deleted cells dissociated from primary tracheospheres (N=3).

(C) Relative number of primary tracheospheres formed by WT or Trp53-deleted mouse tracheal epithelial cells (N=3).

(D) Relative number of secondary tracheospheres formed by WT or Trp53-deleted cells dissociated from primary tracheospheres (N=3).

(E) Relative number of tracheospheres from wild type, Keap1−/−, Trp53−/−, and Keap1−/−;Trp53−/− tracheal epithelial cells. Data are presented as mean ± S.E.M. (N=5 for WT and Keap1−/−,N=3 for Trp53−/−, and N=4 for Keap1−/−;Trp53−/−). All data in (A–E) are presented as mean ± S.E.M. (* P < 0.05, ** P < 0.01).

(F) Brightfield images of tracheospheres initiated by WT, Keap1−/−, Trp53−/−, and Keap1−/−;Trp53−/− tracheal epithelial cells.

(G) H&E staining of wild type, Keap1−/−, Trp53−/− and Keap1−/−;Trp53−/− tracheospheres. All scale bars = 100 μm.