Figure 1.

Lysosomes Contribute to bpAP-Evoked Dendritic Ca²⁺ Signaling

(A) Top: CA1 neuron loaded with Alexa 488 (green) and stained with LysoTracker (red) (scale bar, 10 μm). Disruption of the lysosomal membrane with GPN abolished LysoTracker staining. Regions of interest (white boxes) are magnified below (scale bar, 2 μm).

(B) Average LysoTracker staining in ACSF (artificial cerebrospinal fluid) and GPN (n = 5 cells/condition). Significance was assessed with a Mann-Whitney test.

(C) Example bpAP-evoked Ca²⁺ transient in CA1 apical dendrites loaded with OGB-1 (scale bar, 10 μm). Laser scanning was restricted to a line across a region of interest (circle). The resulting line scan (scale bar, 50 ms), along with its quantification (%ΔF/F) is shown time-locked with electrophysiological recordings. GPN reduced the bpAP-evoked Ca²⁺ influx.

(D) CA1 neuron loaded with OGB-1. Ca²⁺ transients evoked before (black traces) and after (red traces) the addition of GPN are shown for regions of interest (scale bar, 20 μm).

(E) Average data (n = 7–18 cells/condition).

Significance was assessed with Kruskal-Wallis and post hoc Dunn’s tests. Error bars represent SEM. See also Figures S1 and S2.