Figure 2.

Lysosomal Ca²⁺ Release Requires NAADP

(A–D) Left: sample Ca²⁺ transients evoked by single bpAPs in CA1 apical dendrites. Ca²⁺ transients were reduced following inhibition of lysosomal Ca²⁺ signaling by various reagents. Right: average peak Ca²⁺ signal measured across conditions (n = 5–10 cells/condition). Significance was assessed with Mann-Whitney tests (single comparisons) or Kruskal-Wallis and post hoc Dunn’s tests (multiple comparisons).

(E) Left: example of line scan images (scale bar, 100 ms) and quantified traces (%ΔF/F) of Ca²⁺ signals recorded in CA1 apical dendrites in acute hippocampal slices. Right: average peak fluorescence (n = 6–7 cells/condition). Ca²⁺ transients were reduced by GPN and NED-19. Significance was assessed with Kruskal-Wallis and post hoc Dunn’s tests.

Error bars represent SEM. See also Figures S1–S5.