Figure 1.
Differential mNET-Seq Profiles for Protein Coding and lincRNA genes
(A) mNET-seq strategy with each Pol II phospho-CTD modification color coded.
(B and C) Color-coded heatmaps showing phospho-CTD profiles across individual (B) protein coding TUs and (C) lincRNA TUs ordered based on their transcription levels. Profiles are aligned to TSS and TES as indicated. Genes >1,000 nt (excluding some smaller protein coding and lincRNA genes) were divided into 100 bins.
(D and E) (D) mNET-seq profiles across TARS (black for protein-coding gene) and (E) LINC01021 (green for lincRNA gene) using seven different Pol II antibodies as indicated. Gene maps show exons filled in and introns hatched. A chromatin-seq profile is run below the mNET-seq profiles. Blue reads are sense and red reads antisense transcripts. Reads per 108 mapped reads are indicated in brackets.
See also Figure S1.