Table 1.

Polymer characterization, antimicrobial activity of polymers, chlorhexidine (CHX), and vancomycin (VAN) against planktonic S. mutans, and hemolytic activity.

Polymer/antimicrobial	MPethyla (mol. %)	DPb	Mn (NMR)c	Đd	MICe (μg/mL)	MBCf (μg/mL)	HC50g (μg/mL)
			2,400				>1000
PE0	0	13.9		1.32	52.1 ± 14.7	62.5 ± 0.0
			(4,000)				(0.9 ± 0.1%)
			2,500				>1000
PE31	30.6	15.9		1.12	7.8 ± 0.0	10.4 ± 3.7
			(3,800)				(26.9 ± 9.6%)
CHXh	–	–	626	–	0.3 ± 0.1	4.2 ± 2.5	133 ± 23.6
VANi	–	–	1,486	–	0.8 ± 0.0	2.3 ± 1.1	n.d.j

^aMP_ethyl: Mole percentage of ethyl group in a polymer chain determimed by ¹H NMR.

^bDP: The number average degree of polymerization determimed by ¹H NMR.

^cM_n (NMR): The number average molecular weight (M_n) without trifluoroacetate calculated based on the molecular weight of monomers, MP_methyl and DP. The molecular weight including trifluoroacetate is given in parenthesis.

^dĐ: The dispersity of boc-protected polymers calculated as M_w/M_n using M_w and M_n values determined by GPC.

^eMIC: minimum inhibitory concentration.

^fMBC: minimum bactericidal concentration, the lowest concentration to reduce viable bacterial cells less than 1000 cfu/mL.

^gHC₅₀: concentration causing 50% lysis (hemolysis) of red blood cells (RBCs) relative to Trinton-X (positive control, 100 %) and solvents (negative control, 0.001% acetic acid for PE₀, PE₃₁ and VAN and water for CHX, 0 %). The hemolysis % at 1000 μg/mL is given in parenthesis.

^hChlorhexidine diacetate.

ⁱVancomycin hydrochloride.

^jNot determined.